DNA platform
Streamlined development solutions for plasmids and libraries for viral vector and mRNA therapeutics.
Precise variant control and representation of codons for protein expressing sequences. Specify an exact combination of codons, a precise percentage of desired amino acids and reduce frameshift mutations for the best mutant integrity.
Or introduce singular mutations in a user-defined region or truncate a sequence to characterize and optimize the desired protein features and function.
A powerful tool for determining and optimizing biomolecule function from a parent sequence.
Create a library of randomized, partially randomized or specifically designated mutants with multiple, different combinations of genetic elements. Precisely create codon or DNA part variants in combination to efficiently explore the vast design space.
Design DNA for functionality while we handle the construction. With DNA construct synthesis up to 50kbp and none of the complexity limits of traditional manufacture methods, you concentrate on design while we bring your creations to life.
CDS libraries
Precise variant control and representation of codons for protein expressing sequences. Specify an exact combination of codons, a precise percentage of desired amino acids (as low as 5%) and reduce frameshift mutations for the best mutant integrity. Or introduce singular/scanning site-directed mutagenesis in a user-defined region or truncate a sequence to characterize and optimize the desired protein features and function.
Combinatorial Libraries
A powerful tool for determining and optimizing biomolecule function from a parent sequence.
Create a library of completely randomized (NNN) or partially randomized (NNK/NNS) mutants with multiple, different combinations of genetic elements. Precisely create codon or DNA part variants in combination to efficiently explore the vast design space.
Complex Construct Synthesis
Design DNA for functionality while we handle the construction. With DNA construct synthesis up to 50kbp and none of the complexity limits of traditional manufacture methods, you concentrate on design while we bring your creations to life.
Complex design as standard
Explore a near limitless sequence space with industry leading standards on GC content (15-85%), long stretches of repeating sequences and homopolymers (150bp),
Work with collections
Mix and match de novo synthesis of your target sequences with in vivo verified parts from our collections. Or synthesise, store and reuse your own frequently used parts. That way you can save time and budget on your projects.
Precise Codon and Amino Acid representation
Control precisely the codon and amino acid representation in your library to match requirements for your research. Our precision synthesis services lets you balance or bias representation as needed.
Highly Diverse, Highly Complex
Our precision DNA synthesis platform allows you to explore the entire sequence space, without compromise.
Library diversity upto 10^9 and capabilities to produce highly complex libraries with variants across multiple non-contiguous sequences of interest effectively multiplies your discovery potential.
We deliver constructs as lyophilised DNA with options from research grade to endotoxin-free purity at milligram scales and linear or circular form.
Mutant libraries can be delivered as mixed pool of variants or as individual subpools.
Cloned Libraries
- Up to 5mg of plasmid (high copy)
Linear DNA and RNA libraries
- 500 ng-1 µg
Libraries can be delivered into your own custom destination vector or one of our own standard destination vectors suitable for your downstream screening protocols.
We enable the precise encoding of amino acid variants.
Uniform Amino Acid representation:
For example, we can manage uniform codon usage schemes for example G125 to 25% A, 25% V, 25% L, 25% Q),
Biased Amino Acid representation:
We can also provide more complex, biased representation schemes for example G125 to 60% A, 20% V, 20% L.
Codon Optimisation and Representation:
We can optimise coding sequences for your target organism of interest or control precisely the balance of codon usage in your library to support discovery goals.
Degenerate Codon Libraries:
We can also provide the following degenerate libraries:
Degenerate codon | No. of codons | No. of amino acids | No. of stops | Amino acids encoded |
---|---|---|---|---|
NNN | 64 | 20 | 3 | All 20 |
NNK / NNS | 32 | 20 | 1 | All 20 |
NDT | 12 | 12 | 0 | RNDCGHILFSYV |
DBK | 18 | 12 | 0 | ARCGILMFSTWV |
NRT | 8 | 8 | 0 | RNDCGHSY |
We offer both next-generation sequencing (NGS) and Sanger sequencing for construct sequence verification and to verify library coverage and diversity.
- For variants we guarantee 100% sequence verification through sanger sequencing
- Internal quality standards ensure over 90% coverage for NGS sequencing of libraries
Upon delivery of your libraries we will provide:
- Certificate of Analysis
- Batch documentation
Our foundry capabilities allow for in vitro RNA transcription from any DNA template to eliminate traditional constraints faced in research.
High fidelity, purity, and yield allows us to cater to a wide range of research applications. Whether you're delving into expression studies, protein translation or other RNA centric research questions.
Explore and optimise regulatory sequences with in vitro verified DNA parts and targeted sequence variants of 3' and 5' untranslated regions.
RNA transcription
Our foundry capabilities allow for in vitro RNA transcription from any DNA template to eliminate traditional constraints faced in research.
High fidelity, purity, and yield allows us to cater to a wide range of research applications. Whether you're delving into expression studies, protein translation or other RNA centric research questions.
UTR Libraries
Explore and optimise regulatory sequences with in vitro verified DNA parts and targeted sequence variants of 3' and 5' untranslated regions.
Long, complex templates
Explore a near limitless sequence space with industry leading standards on GC content (15-85%), long stretches of repeating sequences and homopolymers (150bp) on CDS up to 10kbp.
Modular Assembly protocols
Elevate your mRNA research with Officinae Bio’s cutting-edge Modular Assembly Protocols by combining in vivo verified part sequences and your own frequently used collections with de novo synthesis.
Explore variants of your transcript with collections of verified promoters and UTRs. Or quickly reuse parts from your own collection that you synthesise and store with us for more effective turnaround.
Precise control over Poly A tails
Case Study: Combinatorial pathway optimization
We built a combinatorial library for metabolic pathway optimisation. This library explored various elements such as enhancers, repressors, promoters, UTRs, binding sites, localization signals, genes, terminators, and barcodes to fine-tune and maximize the production of the target product.
Case Study: Mastering sophisticated randomization schemes
We delivered a fully customized library comprising 18.1 million variants. These variants were generated by incorporating user-defined codon usage patterns, which are distributed across multiple non-contiguous positions.
18.1 million variants.
25 days delivery.
Combinatorial Libraries to fine tune mRNA expression
Leverage our sequential approach to optimising mRNA expression for a cost-effective approach to your mRNA construct designs.
We build the tools needed for nucleic acid manufacture so you can focus on creating life changing therapeutics.
-----------------------
At Officinae Bio, we're developing a platform to support the future of biologically designed therapeutics. We don't ignore biology's complexity, we embrace it. By developing breakthroughs in AI-enabled methodologies for DNA manufacture, biological optimisation and synthetic biology, we help you develop the next generation in advanced therapeutics.